pSG7163 pLIBT7_SNAPtag-3C-8His
(Plasmid
#232622)
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PurposeExpression vector for SNAPtag (carboxy terminal 3C-8His tag)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLIBT7
- Backbone size w/o insert (bp) 5151
- Total vector size (bp) 5726
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAPtag-3C-8His
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SpeciesSynthetic
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Insert Size (bp)596
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Tag
/ Fusion Protein
- 3C-8His (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains AVK312-314TGG mutations in LacI. These mutations are not known to impact plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG7163 pLIBT7_SNAPtag-3C-8His was a gift from Stephan Gruber (Addgene plasmid # 232622 ; http://n2t.net/addgene:232622 ; RRID:Addgene_232622)