pUC19_CDC45_dTAG
(Plasmid
#232733)
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PurposeGenerates CDC45 dTAG C ternimal knock-in cell lines as donor
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCDC45
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SpeciesH. sapiens (human)
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Insert Size (bp)2718
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MutationSynonymous mutation
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Entrez GeneCDC45 (a.k.a. CDC45L, CDC45L2, MGORS7, PORC-PI-1)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's NGS sequence contains 3 SNPs in the right arm when compared to the reference; these do not have any effect on the plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19_CDC45_dTAG was a gift from Junjie Chen (Addgene plasmid # 232733 ; http://n2t.net/addgene:232733 ; RRID:Addgene_232733)