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Addgene

ATP5F1B-pmCherry
(Plasmid #232783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP5F1B
  • Alt name
    ATP5B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1587
  • Entrez Gene
    ATP5F1B (a.k.a. ATP5B, ATPMB, ATPSB, HEL-S-271, HUMOP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATP5F1B-pmCherry was a gift from Giulia Bertolin (Addgene plasmid # 232783 ; http://n2t.net/addgene:232783 ; RRID:Addgene_232783)

  • For your References section:

    Aurora kinase A/AURKA functionally interacts with the mitochondrial ATP synthase to regulate energy metabolism and cell death. Sharma RK, Chafik A, Bertolin G. Cell Death Discov. 2023 Jun 29;9(1):203. doi: 10.1038/s41420-023-01501-2. 10.1038/s41420-023-01501-2 PubMed 37386025
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