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pAM-CaMKII-EGFP-T2A-FlipO
(Plasmid #232791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAM
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CaMKII-EGFP-T2A-FlipO
  • Species
    Synthetic

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TCTGCTTTCTCACCCCTA
  • 3′ sequencing primer gcgtaaaaggagcaacata
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Matthias Klugmann (Mol. Cell. Neurosci. 2005, 28:343-360 https://doi.org/10.1016/j.mcn.2004.10.002), Andrew Murray (Nat. Neurosci. 2011 Mar;14(3):297-9. doi: 10.1038/nn.2751).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Authors were not aware of the 493 base pair deletion in the backbone, which includes a part of ITR. They were able to package the plasmid into AAV1/2 capsids, however, since mutations of ITRs can lead to reduced efficiency in viral packaging, they recommend to reclone the construct into an AAV vector with intact ITRs.

The AAV backbone containing the 1.1-kb CMV enhancer/chicken h-actin (CBA) promoter, the woodchuck posttranscriptional regulatory element (WPRE), and the bovine growth hormone polyA was derived from a plasmid made by Matthias Klugmann (Mol. Cell. Neurosci. 2005, 28:343-360 https://doi.org/10.1016/j.mcn.2004.10.002)

Please visit https://doi.org/10.1101/2024.08.13.606964 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM-CaMKII-EGFP-T2A-FlipO was a gift from Peer Wulff (Addgene plasmid # 232791 ; http://n2t.net/addgene:232791 ; RRID:Addgene_232791)

  • For your References section:

    Parvalbumin expression identifies subicular principal cells with high projection specificity. Baccini G, Foggetti A, Wernet N, Engelhardt KA, Kronenbitter K, Michels J, Kulik A, Wozny C, Wulff P. bioRxiv 2024.08.13.606964 10.1101/2024.08.13.606964
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