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pETDuet-larA-strep-larB
(Plasmid #232792)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232792 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pETDuet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 7241
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Lactate racemase
  • Alt name
    larA
  • Species
    Lactiplantibacillus plantarum
  • Insert Size (bp)
    1275
  • GenBank ID
    AL935263
  • Tag / Fusion Protein
    • Strep-tag II (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CTTTAAGAAGGAGATATACCATGTCCGTTGCAATTGATTTACCATATGACAA
  • 3′ sequencing primer CCGCAAGCTTGTCGACCTACTTCTCAAATTGTGGATGACTCCAGC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Pyridinium-3,5-biscarboxylic acid mononucleotide synthase
  • Alt name
    larB
  • Species
    Lactiplantibacillus plantarum
  • Insert Size (bp)
    738
  • GenBank ID
    AL935263

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GTTAAGTATAAGAAGGAGATATACATATGGCAACCACAGCAGAAATATTACAACAAGTG
  • 3′ sequencing primer CCAGACTCGAGGGTACCTTACATTTGATTGACCATACTAGCTGAGTAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-larA-strep-larB was a gift from Robert Hausinger (Addgene plasmid # 232792 ; http://n2t.net/addgene:232792 ; RRID:Addgene_232792)

  • For your References section:

    Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes. Nevarez JL, Turmo A, Gatreddi S, Gupta S, Hu J, Hausinger RP. mBio. 2024 Dec 16:e0340424. doi: 10.1128/mbio.03404-24. 10.1128/mbio.03404-24 PubMed 39679682
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