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Addgene

YpFabG_pET-28a(+)-TEV
(Plasmid #232927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)-TEV
  • Total vector size (bp) 1124
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    3-oxoacyl-[acyl-carrier-protein] reductase
  • Alt name
    FabG
  • Species
    Y. pestis
  • Insert Size (bp)
    800
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer GCT AGT TAT TGC TCA GCG G
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YpFabG_pET-28a(+)-TEV was a gift from Josh Beckham (Addgene plasmid # 232927 ; http://n2t.net/addgene:232927 ; RRID:Addgene_232927)
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