pNK036C_AttB_mkozak_mGreenLantern-STIM1_IRES_mCherry-H2A-P2A-PuroR
(Plasmid
#232938)
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PurposeLow expression plasmid of STIM1 with N-terminal mGreenLantern tag for Matreyek Bxb1 (GT) landing pad
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232938 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneBxb1 attB recombination vector
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Backbone manufacturerMatreyek Lab
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSTIM1
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SpeciesH. sapiens (human)
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Entrez GeneSTIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
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Tag
/ Fusion Protein
- mGreenLantern (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySTIM1; pLNCX2-STIM1 was a gift from Shengyu Yang (Addgene plasmid # 89817 ; http://n2t.net/addgene:89817 ; RRID:Addgene_89817)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.01.23.634525 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNK036C_AttB_mkozak_mGreenLantern-STIM1_IRES_mCherry-H2A-P2A-PuroR was a gift from Kenneth Matreyek (Addgene plasmid # 232938 ; http://n2t.net/addgene:232938 ; RRID:Addgene_232938) -
For your References section:
Comprehensive mutational characterization of the calcium-sensing STIM1 EF-hand reveals residues essential for structure and function. Kamath ND, Matreyek KA. Genetics. 2025 Jul 30:iyaf146. doi: 10.1093/genetics/iyaf146. 10.1093/genetics/iyaf146 PubMed 40737484