pSC6
(Plasmid
#233020)
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Purpose(Empty Backbone) Inducible Conjugative Plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233020 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSC5GGv1
- Backbone size (bp) 55757
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Modifications to backboneDeletion of pSC5GGv1 ΔtrbF gene. Insertion of trbF gene and mRFP driven by arabinose inducible pBAD promoter and terminator.
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Epi300 (Epicentre)
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Copy numberLow Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSC6 was a gift from Bogumil Karas (Addgene plasmid # 233020 ; http://n2t.net/addgene:233020 ; RRID:Addgene_233020) -
For your References section:
Engineering conjugative plasmids for inducible horizontal DNA transfer. Jaafar T, Carvalhais E, Shrestha A, Cochrane R, Meaney J, Brumwell S, Hamadache S, Nasrollahi V, Karas BJ. Can J Microbiol. 2025 May 6;71:1-9. doi: 10.1139/cjm-2024-0241. 10.1139/cjm-2024-0241 PubMed 40168690