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Addgene

pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA
(Plasmid #233045)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233045 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAK
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
  • Species
    Synthetic
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pAK_DR30_CasRx_Puro, Addgene plasmid #134845

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA was a gift from Jonathan Ploski (Addgene plasmid # 233045 ; http://n2t.net/addgene:233045 ; RRID:Addgene_233045)

  • For your References section:

    Adeno-Associated Viral Vector Resource for the RNA-Targeting Cas13d: A Comparison of High-Fidelity Variants, DjCas13d and hfCas13d. Back F, Sandoval A, Vu L, Hong VM, Bhaskara A, Rodriguez SR, O’Brien JT, Kolber BJ, Kroener S, Ploski JE. Mol Ther Methods Clin Dev, 20 Aug 2025, 101565 10.1016/j.omtm.2025.101565
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