pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE
(Plasmid
#233047)
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PurposeTo express GFP from an EFS promoter in the 3'-5' direction and Flag-GluN2B from a TRE3g promoter in the 5'-3' direction
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP and GluN2B
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SpeciesSynthetic
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE was a gift from Jonathan Ploski (Addgene plasmid # 233047 ; http://n2t.net/addgene:233047 ; RRID:Addgene_233047)
