pHR-Flag-IF1-Fibcon-icam
(Plasmid
#233076)
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Purposeexpresses synCAM -IF1-fibcon - iCAM1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Backbone manufacturerLim Lab, Addgene 79121
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIf1-Icam
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byIF1 from Derek Woolfson's lab, Rhys et al. 2018 (https://doi.org/10.1038/s41467-018-06391-y). Fibcon from Karyn T O'Neil's lab, Jacobs et al. 2012 (https://doi.org/10.1093/protein/gzr064).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-Flag-IF1-Fibcon-icam was a gift from Wendell Lim (Addgene plasmid # 233076 ; http://n2t.net/addgene:233076 ; RRID:Addgene_233076) -
For your References section:
Programming multicellular assembly with synthetic cell adhesion molecules. Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA. Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z. Epub 2022 Dec 12. 10.1038/s41586-022-05622-z PubMed 36509107