pVKS-1
(Plasmid
#233086)
-
PurposeHNRNPD full-length promoter reporter construct
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 233086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepGL3-Basic
-
Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 6521
-
Vector typeLuciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer pGL-Forward (5ʹ-CTAGCAAAATAGGCTGTCCC-3ʹ)
- 3′ sequencing primer pGL-Reverse (5ʹ-CTTTATGTTTTTGGCGTCTTCC-3ʹ) (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pVKS-1 was a gift from Shyam Chauhan (Addgene plasmid # 233086 ; http://n2t.net/addgene:233086 ; RRID:Addgene_233086) -
For your References section:
NFkappaB (RelA) mediates transactivation of hnRNPD in oral cancer cells. Kumar V, Kumar A, Kumar M, Lone MR, Mishra D, Chauhan SS. Sci Rep. 2022 Apr 8;12(1):5944. doi: 10.1038/s41598-022-09963-7. 10.1038/s41598-022-09963-7 PubMed 35396527