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Addgene

pVKS-1
(Plasmid #233086)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233086 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 6521
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNRNPD
  • Alt name
    AUF-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1663
  • Entrez Gene
    HNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
  • Entrez Gene
    AUF1 (a.k.a. AUF1A)
  • Promoter HNRNPD Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer pGL-Forward (5ʹ-CTAGCAAAATAGGCTGTCCC-3ʹ)
  • 3′ sequencing primer pGL-Reverse (5ʹ-CTTTATGTTTTTGGCGTCTTCC-3ʹ)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVKS-1 was a gift from Shyam Chauhan (Addgene plasmid # 233086 ; http://n2t.net/addgene:233086 ; RRID:Addgene_233086)

  • For your References section:

    NFkappaB (RelA) mediates transactivation of hnRNPD in oral cancer cells. Kumar V, Kumar A, Kumar M, Lone MR, Mishra D, Chauhan SS. Sci Rep. 2022 Apr 8;12(1):5944. doi: 10.1038/s41598-022-09963-7. 10.1038/s41598-022-09963-7 PubMed 35396527
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