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Addgene

pYihI
(Plasmid #233088)

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Item Catalog # Description Quantity Price (USD)
Plasmid 233088 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pGEX4T1
  • Backbone size w/o insert (bp) 4958
  • Total vector size (bp) 5465
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YihI
  • Species
    E. coli
  • Insert Size (bp)
    507
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.12.637445 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYihI was a gift from Michael Downey (Addgene plasmid # 233088 ; http://n2t.net/addgene:233088 ; RRID:Addgene_233088)

  • For your References section:

    Identification of polyphosphate-binding proteins in E. coli uncovers targets involved in translation control and ribosome biogenesis. Baijal K, Kore B, Abramchuk I, Denoncourt A, Han S, Simms A, Dagenais A, Long AR, Rudner AD, Lavallée-Adam M, Gray MJ, Downey M. bioRxiv 2025.02.12.637445 10.1101/2025.02.12.637445
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