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Addgene

pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1)
(Plasmid #233270)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233270 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-MCS AAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP and shRNA targeting Rat NPAS4
  • gRNA/shRNA sequence
    Unknown
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Npas4 (a.k.a. Nxf)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SapI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1) was a gift from Jonathan Ploski & Glenn Schafe (Addgene plasmid # 233270 ; http://n2t.net/addgene:233270 ; RRID:Addgene_233270)

  • For your References section:

    The neuronal PAS domain protein 4 (Npas4) is required for new and reactivated fear memories. Ploski JE, Monsey MS, Nguyen T, DiLeone RJ, Schafe GE. PLoS One. 2011;6(8):e23760. doi: 10.1371/journal.pone.0023760. Epub 2011 Aug 22. 10.1371/journal.pone.0023760 PubMed 21887312
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