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pDsRed1-CMV-Rat Npas4-DsRed1-pA
(Plasmid #233272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pdsRedN-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rat Npas4 DsRed fusion
  • Species
    R. norvegicus (rat)
  • Mutation
    Y2D
  • Entrez Gene
    Npas4 (a.k.a. Nxf)
  • Tag / Fusion Protein
    • DsRed1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Be advised the NPAS4 coding region contains a Y2D mutation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDsRed1-CMV-Rat Npas4-DsRed1-pA was a gift from Jonathan Ploski & Glenn Schafe (Addgene plasmid # 233272 ; http://n2t.net/addgene:233272 ; RRID:Addgene_233272)

  • For your References section:

    The neuronal PAS domain protein 4 (Npas4) is required for new and reactivated fear memories. Ploski JE, Monsey MS, Nguyen T, DiLeone RJ, Schafe GE. PLoS One. 2011;6(8):e23760. doi: 10.1371/journal.pone.0023760. Epub 2011 Aug 22. 10.1371/journal.pone.0023760 PubMed 21887312
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