pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #5)
(Plasmid
#233274)
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PurposeTo express GFP from a CMV promoter and to express and shRNA targeting Rat Nurr1
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233274 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-MCS AAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP and shRNA targeting Rat NPAS1
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gRNA/shRNA sequenceUnknown
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SpeciesR. norvegicus (rat)
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Entrez GeneNpas1
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SapI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #5) was a gift from Jonathan Ploski & Glenn Schafe (Addgene plasmid # 233274 ; http://n2t.net/addgene:233274 ; RRID:Addgene_233274)