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pDG459-HNRNPD-KO
(Plasmid #233287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233287 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDG459
  • Backbone manufacturer
    Paul Thomas modified PX459 (Plasmid #100901)
  • Backbone size w/o insert (bp) 9579
  • Total vector size (bp) 9586
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNRNPD
  • Alt name
    AUF-1
  • gRNA/shRNA sequence
    EXON1 (5'-CGCCGAACTGCTCCTCCGAC-3') & EXON2 (5'-CTGAAGCAGCGACGGCACAG-3')
  • Species
    H. sapiens (human)
  • Entrez Gene
    HNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
  • Entrez Gene
    AUF1 (a.k.a. AUF1A)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer pDG459 Seq1 (5'-TTTGTCTGCAGAATTGGC-3')
  • 3′ sequencing primer pDG459 Seq2 (5'-CTCTAGAACTAGTGGATC-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.05.631360 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDG459-HNRNPD-KO was a gift from Shyam Chauhan (Addgene plasmid # 233287 ; http://n2t.net/addgene:233287 ; RRID:Addgene_233287)
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