AAV-EF1α-loxP-GCaMP6f-loxP-WPRE
(Plasmid #233288)

• Purpose: AAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.
• Depositing Lab: Dayu Lin
• Publication: Fang et al Neuron. 2018 Apr 4;98(1):192-207.e10. doi: 10.1016/j.neuron.2018.02.019.

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Backbone

• Vector backbone: AAV-EF1α-loxP-loxP-WPRE
• Total vector size (bp): 6825
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GCaMP6f
• Species: Synthetic
• Insert Size (bp): 1353
• Promoter: EF1α

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: EF1α
• 3′ sequencing primer: WPRE

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry