pET29b_SpyTag003-L-VHH21-His
(Plasmid
#233333)
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PurposeTo express nanobody VHH21 with N terminal SpyTag
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET_29b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpyTag003-L-VHH21-His
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SpeciesSynthetic
- Promoter T7
Cloning Information
- Cloning method Gene Synthesis
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29b_SpyTag003-L-VHH21-His was a gift from Magnus Rueping (Addgene plasmid # 233333 ; http://n2t.net/addgene:233333 ; RRID:Addgene_233333) -
For your References section:
Nanobodies for netosis tracking and visualization. Gutu N, Peng Y, Renn D, Rueping M. Current Protocols, 2025, 5, e70213 10.1002/cpz1.70213