pMRX-IB-FKBP-HaloTag
(Plasmid
#233350)
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PurposeStable expression of FKBP-Halotag in mammalian cells. FKBP-halotag localizes in the cytosol and binds to the FRB tag in the presence of AP21967 (A/C Heterodimerizer).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMRX-IB
- Total vector size (bp) 7133
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsDH5alpha will also be fine
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKBP
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Insert Size (bp)324
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Tag
/ Fusion Protein
- HaloTag7 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.08.13.607738 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRX-IB-FKBP-HaloTag was a gift from Noboru Mizushima (Addgene plasmid # 233350 ; http://n2t.net/addgene:233350 ; RRID:Addgene_233350) -
For your References section:
Optogenetic tools for inducing organelle membrane rupture. Nagashima Y, Eguchi T, Koyama-Honda I, Mizushima N. J Biol Chem. 2025 Mar 18;301(4):108421. doi: 10.1016/j.jbc.2025.108421. 10.1016/j.jbc.2025.108421 PubMed 40113041