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Addgene

pMTKM03
(Plasmid #233475)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233475 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBK01
  • Backbone manufacturer
    Vincent Martin, Addgene plasmid #178985
  • Vector type
    Yeast Expression, CRISPR, Synthetic Biology
  • Selectable markers
    KanamycinR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The origin of replication from the original backbone was replaced with a pan-yeast autonomously replicating sequence (ARS) from PMID: 24205893.

Please visit https://doi.org/10.1101/2025.02.03.636257 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMTKM03 was a gift from Vincent Martin (Addgene plasmid # 233475 ; http://n2t.net/addgene:233475 ; RRID:Addgene_233475)

  • For your References section:

    Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a Host for Lactose Valorization. Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, Martin VJJ. ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096. 10.1021/acssynbio.5c00096 PubMed 40629255
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