pET26b-BsaI
(Plasmid
#233523)
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Purpose(Empty Backbone) Purification vector with BsaI cut sites for streamlined cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET26b_Nb.b201
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Backbone manufacturerAndrew Kruse
- Backbone size (bp) 5656
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Modifications to backboneRemoval of Nb.b201 sequence, replaced with MCS containing BsaI restriction enzyme cut sites
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Vector typeBacterial Expression
- Promoter T7
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Tags
/ Fusion Proteins
- pelB leader (N terminal on backbone)
- 6xHis-tag (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer T7 Foward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET26b-BsaI was a gift from Carl Denard (Addgene plasmid # 233523 ; http://n2t.net/addgene:233523 ; RRID:Addgene_233523)
