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pCambia1300_cLUC-NLRb
(Plasmid #233527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233527 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCambia1300-cLUC
  • Backbone manufacturer
    Zhao and Zhou 2020, doi: https://doi.org/10.11983/CBB19229
  • Backbone size w/o insert (bp) 10935
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sr62NLRb
  • Species
    Triticum aestivum
  • Insert Size (bp)
    3117
  • Promoter 35S
  • Tag / Fusion Protein
    • C-terminal fragment of Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KnpI (not destroyed)
  • 3′ cloning site SalI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.30.610287 for bioRxiv preprint.

A E180D mutation was found in HygR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCambia1300_cLUC-NLRb was a gift from Brande Wulff (Addgene plasmid # 233527 ; http://n2t.net/addgene:233527 ; RRID:Addgene_233527)

  • For your References section:

    A wheat tandem kinase sensor activates an NLR helper to trigger immunity. Chen R, Chen J, Outram MA, Powell OR, Arndell T, Gajendiran K, Wang YL, Ayliffe MA, Blundell C, Figueroa M, Sperschneider J, Vanhercke T, Tang D, Xu Y, Zhong G, Gardener C, Yu G, Gourdoupis S, Jaremko Ł, Matny O, Steffenson BJ, Boshoff WHP, Meyer WB, Arold ST, Dodds PN, Wulff BBH. bioRxiv 2024.08.30.610287 10.1101/2024.08.30.610287
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