[#GS24 -LV] Syn1-GFP-OMP25
(Plasmid
#233566)
-
PurposeLentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 233566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLenSyn1
-
Backbone manufacturerModifed from pLenSyn2-EGFP-OMP25C, Henri Huttunen, Addgene plasmid #71427
- Total vector size (bp) 8298
-
Vector typeMammalian Expression, Lentiviral, Synthetic Biology
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameeGFP
-
SpeciesSynthetic; Aequorea victoria
-
Entrez GeneeGFP (a.k.a. pPRS3a_01)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Nanopore (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byPlasmid derived from Addgene #71427
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
[#GS24 -LV] Syn1-GFP-OMP25 was a gift from Simon Grelet (Addgene plasmid # 233566 ; http://n2t.net/addgene:233566 ; RRID:Addgene_233566) -
For your References section:
Nerve-to-cancer transfers of mitochondria during cancer metastasis. Hoover G, Gilbert S, Curley O, Obellianne C, Lin MT, Hixson W, Pierce TW, Andrews JF, Alexeyev MF, Ding Y, Bu P, Behbod F, Medina D, Chang JT, Ayala G, Grelet S.. Nature. 2025; In Press.