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Addgene

pcDNA5/FRT/TO-3xFLAG-His6-LARP1-d327-496
(Plasmid #233627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LARP1delta327-496
  • Species
    H. sapiens (human)
  • Mutation
    delta327-496
  • Entrez Gene
    LARP1 (a.k.a. LARP, Lar1, Lhp1)
  • Tag / Fusion Protein
    • 3FLAG-His6 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO-3xFLAG-His6-LARP1-d327-496 was a gift from Shintaro Iwasaki (Addgene plasmid # 233627 ; http://n2t.net/addgene:233627 ; RRID:Addgene_233627)

  • For your References section:

    eIF4A1 enhances LARP1-mediated translational repression during mTORC1 inhibition. Shichino Y, Yamaguchi T, Kashiwagi K, Mito M, Takahashi M, Ito T, Ingolia NT, Kuba K, Iwasaki S. Nat Struct Mol Biol. 2024 Oct;31(10):1557-1566. doi: 10.1038/s41594-024-01321-7. Epub 2024 May 21. 10.1038/s41594-024-01321-7 PubMed 38773334
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