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AAV-Grik1-GFP
(Plasmid #233645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV-RedO-GFP
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Grik1 promoter
  • Alt name
    GLUR5
  • Alt name
    GluK1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    730
  • Entrez Gene
    Grik1 (a.k.a. A830007B11Rik, D16Ium24, D16Ium24e, GluK1, GluK5, Glur-5, Glur5, Glurbeta1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BmtI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned at Cepko Lab by Sean K. Wang based on a Grik1 enhancer previously identified by Sylvain W. Lapan (PMID: 31110282).

Please visit https://doi.org/10.1101/2023.11.02.565326 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-Grik1-GFP was a gift from Connie Cepko (Addgene plasmid # 233645 ; http://n2t.net/addgene:233645 ; RRID:Addgene_233645)

  • For your References section:

    ERG responses to high-frequency flickers require FAT3 signaling in mouse retinal bipolar cells. Avilés EC, Wang SK, Patel S, Cordero S, Shi S, Lin L, Kefalov VJ, Goodrich LV, Cepko CL, Xue Y. J Gen Physiol. 2025 Mar 3;157(2):e202413642. doi: 10.1085/jgp.202413642. Epub 2025 Feb 4. 10.1085/jgp.202413642 PubMed 39903280
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