AAV-Grik1-GFP
(Plasmid #233645)

• Purpose: Expresses GFP in OFF cone bipolar cells
• Depositing Lab: Connie Cepko
• Publication: Avilés et al J Gen Physiol. 2025 Mar 3;157(2):e202413642. doi: 10.1085/jgp.202413642. Epub 2025 Feb 4.

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Backbone

• Vector backbone: AAV-RedO-GFP
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Grik1 promoter
• Alt name: GLUR5
• Alt name: GluK1
• Species: M. musculus (mouse)
• Insert Size (bp): 730
• Entrez Gene: Grik1 (a.k.a. A830007B11Rik, D16Ium24, D16Ium24e, GluK1, GluK5, Glur-5, Glur5, Glurbeta1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BmtI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned at Cepko Lab by Sean K. Wang based on a Grik1 enhancer previously identified by Sylvain W. Lapan (PMID: 31110282).

Please visit https://doi.org/10.1101/2023.11.02.565326 for bioRxiv preprint.