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Addgene

pRSET-cfp29
(Plasmid #233651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET
  • Backbone size w/o insert (bp) 2754
  • Total vector size (bp) 3552
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab recommends using C41 E. coli for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Encapsulin
  • Alt name
    CFP29
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    798
  • Promoter T7 promoter

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET-cfp29 was a gift from Ye Gao (Addgene plasmid # 233651 ; http://n2t.net/addgene:233651 ; RRID:Addgene_233651)

  • For your References section:

    In situ and in vitro cryo-EM reveal structures of mycobacterial encapsulin assembly intermediates. Berger C, Lewis C, Gao Y, Knoops K, Lopez-Iglesias C, Peters PJ, Ravelli RBG. Commun Biol. 2025 Feb 15;8(1):245. doi: 10.1038/s42003-025-07660-5. 10.1038/s42003-025-07660-5 PubMed 39955411
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