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Addgene

P1909:EVA4_RHISA-StrepII-8xHis
(Plasmid #233698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHLSEC
  • Backbone size w/o insert (bp) 4704
  • Total vector size (bp) 5016
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EVA4
  • Alt name
    Evasin-4
  • Species
    Rhipicephalus sanguineus (tick)
  • Insert Size (bp)
    312
  • Promoter Chicken β actin
  • Tag / Fusion Protein
    • Strep II, 8xHis (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Genewiz

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P1909:EVA4_RHISA-StrepII-8xHis was a gift from Shoumo Bhattacharya (Addgene plasmid # 233698 ; http://n2t.net/addgene:233698 ; RRID:Addgene_233698)

  • For your References section:

    Development of chemokine network inhibitors using combinatorial saturation mutagenesis. Kryukova J, Vales S, Payne M, Smagurauskaite G, Chandra S, Clark CJ, Davies G, Bhattacharya S. Commun Biol. 2025 Apr 3;8(1):549. doi: 10.1038/s42003-025-07778-6. 10.1038/s42003-025-07778-6 PubMed 40181178
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