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pESC-leu2D-LjCPR1-GW
(Plasmid #233735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233735 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pESC-Leu2d
  • Backbone manufacturer
    Stratagene, Jay Keasling
  • Backbone size (bp) 7346
  • Modifications to backbone
    A 2121 bp coding region of LjCPR1 gene (Accession no. AB433810) was inserted at the restriction enzyme sites NotI and PacI. A Gateway cassette (1725 bp) from the pEarleyGate100 vector was inserted at ApaI and SacII.
  • Vector type
    Yeast Expression
  • Promoter Gal 1 and Gal 10
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pESC-leu2D-LjCPR1-GW was a gift from Sangeeta Dhaubhadel (Addgene plasmid # 233735 ; http://n2t.net/addgene:233735 ; RRID:Addgene_233735)

  • For your References section:

    Functional characterization of Cinnamate 4-hydroxylase gene family in soybean (Glycine max). Khatri P, Chen L, Rajcan I, Dhaubhadel S. PLoS One. 2023 May 15;18(5):e0285698. doi: 10.1371/journal.pone.0285698. eCollection 2023. 10.1371/journal.pone.0285698 PubMed 37186600
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