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pGTM_COV2_NSP3_003_SUMO
(Plasmid #233739)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233739 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET15Sumo2_NESG
  • Backbone manufacturer
    Novagen/Northeast Structural Genomics Consortium
  • Backbone size w/o insert (bp) 5737
  • Total vector size (bp) 6691
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PLpro
  • Alt name
    Nsp3
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    954
  • GenBank ID
    WQM42602.1 PRO_0000449637
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter T7 lac
  • Tag / Fusion Protein
    • 6xHis-Sumo (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTM_COV2_NSP3_003_SUMO was a gift from Gaetano Montelione (Addgene plasmid # 233739 ; http://n2t.net/addgene:233739 ; RRID:Addgene_233739)

  • For your References section:

    Protocol for production and characterization of SARS-CoV-2 PL(pro) in Escherichia coli. De Falco A, Greene-Cramer R, Shurina BA, Zakian S, Acton TB, Ramelot TA, Montelione GT. STAR Protoc. 2025 Sep 19;6(3):103952. doi: 10.1016/j.xpro.2025.103952. Epub 2025 Jul 18. 10.1016/j.xpro.2025.103952 PubMed 40682779
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