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Addgene

pRO129
(Plasmid #233805)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233805 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rtTA3
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRO129 was a gift from Jeff Hasty (Addgene plasmid # 233805 ; http://n2t.net/addgene:233805 ; RRID:Addgene_233805)

  • For your References section:

    A Standardized Set of MoClo-Compatible Inducible Promoter Systems for Tunable Gene Expression in Yeast. O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N, Hasty J. ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 10.1021/acssynbio.3c00184. Epub 2023 Dec 11. 10.1021/acssynbio.3c00184 PubMed 38079574
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