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pSecUAG_UTu1D
(Plasmid #233867)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233867 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD-myc-HisA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 7383
  • Modifications to backbone
    RSF origin and spectinomycin resistance were taken from pRSF-Duet1 and used to replace the pBR322 origin and ampicillin resistance of pBAD-myc-HisA
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    allo-tRNA UTu1D
  • Species
    Synthetic
  • Insert Size (bp)
    90
  • GenBank ID
    NC_000019
  • Promoter araBAD

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    thioredoxin
  • Alt name
    Trx1
  • Species
    Treponema denticola
  • Mutation
    Cysteine 32 switched to Selenocysteine (UAG)
  • GenBank ID
    NC_002967

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Selenophosphate synthase
  • Alt name
    SelD
  • Species
    Aeromonas salmonicida
  • Insert Size (bp)
    1038
  • Mutation
    starts with GUG instead of AUG
  • GenBank ID
    NF_002098
  • Promoter native As SelD

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTACCACGGTGCCCAAAAGTC
  • 3′ sequencing primer GCATTTTCGACAGGCTATTG
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    Selenocysteine synthase
  • Alt name
    SelA
  • Species
    Aeromonas salmonicida
  • Insert Size (bp)
    1488
  • GenBank ID
    NF_003308
  • Promoter EM7

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGGGCATTATGCCACAAG
  • 3′ sequencing primer gccgatatactatgccg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSecUAG_UTu1D was a gift from Natalie Krahn & Dieter Söll (Addgene plasmid # 233867 ; http://n2t.net/addgene:233867 ; RRID:Addgene_233867)
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