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pCS2+ Zf nhsl1b-mNeongreen
(Plasmid #233883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233883 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 9637
  • Vector type
    Mammalian Expression ; In vitro synthesis of mRNA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nhsl1b
  • Alt name
    nhsl1
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5546
  • Entrez Gene
    nhsl1b (a.k.a. gukh2, nhsl1, si:ch211-195e3.6)
  • Tag / Fusion Protein
    • mNeongreen (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.01.28.526006 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ Zf nhsl1b-mNeongreen was a gift from Nicolas David (Addgene plasmid # 233883 ; http://n2t.net/addgene:233883 ; RRID:Addgene_233883)

  • For your References section:

    Nance-Horan-syndrome-like 1b controls mesodermal cell migration by regulating protrusion and actin dynamics during zebrafish gastrulation. Escot S, Hassanein Y, Elouin A, Torres-Paz J, Mellottee L, Ignace A, David NB. Commun Biol. 2025 Feb 28;8(1):328. doi: 10.1038/s42003-025-07689-6. 10.1038/s42003-025-07689-6 PubMed 40021913
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