Skip to main content
Addgene

pHRSV_CHARMS-TIR
(Plasmid #233919)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233919 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHR-dSV40
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHARMS-TIR
  • Species
    Synthetic
  • Promoter SV40
  • Tag / Fusion Protein
    • mEGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Created by Gibson assembly using MluI (5') and NotI (3') sites. Please visit https://doi.org/10.1101/2024.05.08.593169 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHRSV_CHARMS-TIR was a gift from Marcus Taylor (Addgene plasmid # 233919 ; http://n2t.net/addgene:233919 ; RRID:Addgene_233919)

  • For your References section:

    Bottom-up reconstruction of functional Death Domain Signalosomes reveals a requirement for polymer stability and avidity. Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E, Taylor MJ. bioRxiv 2024.05.08.593169 10.1101/2024.05.08.593169
Related items: