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pBAD_AIcrVIA1
(Plasmid #234052)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234052 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBAD
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10b is recommended for conducting the phage assays.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AIcrVIA1
  • Species
    Synthetic
  • Promoter araBAD promoter
  • Tag / Fusion Protein
    • 6XHis (C terminal on insert)

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer ATGCCATAGCATTTTTATCC
  • 3′ sequencing primer GATTTAATCTGTATCAGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.05.626932 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD_AIcrVIA1 was a gift from Gavin Knott (Addgene plasmid # 234052 ; http://n2t.net/addgene:234052 ; RRID:Addgene_234052)

  • For your References section:

    De novo design of potent CRISPR-Cas13 inhibitors. Taveneau C, Chai HX, D'Silva J, Bamert RS, Chen H, Hayes BK, Calvert RW, Purcell J, Curwen DJ, Munder F, Martin LL, Barr JJ, Rosenbluh J, Fareh M, Grinter R, Knott GJ. Nat Chem Biol. 2026 Jan 26. doi: 10.1038/s41589-025-02136-3. 10.1038/s41589-025-02136-3 PubMed 41588195
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