AAVS1-SA-CAG-Pa01-BFP
(Plasmid
#234142)
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PurposePerturb Seq landing pad with Pa01 recombinase at the AAVS1 loci
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAVS1_Puro_PGK1_3xFLAG_Twin_Strep
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Vector typeMammalian Expression
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Selectable markersBFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePa01
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gRNA/shRNA sequenceTranscription factors after donor recombination
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Speciessourced from various phage and bacterial genomes, human codon-optomized
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.01.21.633969 for the bioRxiv preprint and find the full Perturb-seq protocol on Protocols.io: https://www.protocols.io/view/experimental-procedures-for-tf-perturb-seq-ut-sout-14egn635pl5d/v1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1-SA-CAG-Pa01-BFP was a gift from Gary Hon (Addgene plasmid # 234142 ; http://n2t.net/addgene:234142 ; RRID:Addgene_234142) -
For your References section:
Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells. Sivakumar S, Wang Y, Goetsch SC, Pandit V, Wang L, Zhao H, Sundarrajan A, Armendariz D, Takeuchi C, Nzima M, Chen WC, Dederich AE, El Hayek L, Gao T, Ghazawi R, Gogate A, Kaur K, Kim HB, McCoy MK, Niederstrasser H, Oura S, Pinzon-Arteaga CA, Sanghvi M, Schmitz DA, Yu L, Zhang Y, Zhou Q, Kraus WL, Xu L, Wu J, Posner BA, Chahrour MH, Hon GC, Munshi NV. bioRxiv [Preprint]. 2025 Jan 23:2025.01.21.633969. doi: 10.1101/2025.01.21.633969. 10.1101/2025.01.21.633969 PubMed 39896670