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AAVS1-SA-CAG-Pa01-BFP
(Plasmid #234142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
  • Vector type
    Mammalian Expression
  • Selectable markers
    BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pa01
  • gRNA/shRNA sequence
    Transcription factors after donor recombination
  • Species
    sourced from various phage and bacterial genomes, human codon-optomized
  • Promoter CAG

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.21.633969 for the bioRxiv preprint and find the full Perturb-seq protocol on Protocols.io: https://www.protocols.io/view/experimental-procedures-for-tf-perturb-seq-ut-sout-14egn635pl5d/v1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-SA-CAG-Pa01-BFP was a gift from Gary Hon (Addgene plasmid # 234142 ; http://n2t.net/addgene:234142 ; RRID:Addgene_234142)

  • For your References section:

    Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells. Sivakumar S, Wang Y, Goetsch SC, Pandit V, Wang L, Zhao H, Sundarrajan A, Armendariz D, Takeuchi C, Nzima M, Chen WC, Dederich AE, El Hayek L, Gao T, Ghazawi R, Gogate A, Kaur K, Kim HB, McCoy MK, Niederstrasser H, Oura S, Pinzon-Arteaga CA, Sanghvi M, Schmitz DA, Yu L, Zhang Y, Zhou Q, Kraus WL, Xu L, Wu J, Posner BA, Chahrour MH, Hon GC, Munshi NV. bioRxiv [Preprint]. 2025 Jan 23:2025.01.21.633969. doi: 10.1101/2025.01.21.633969. 10.1101/2025.01.21.633969 PubMed 39896670
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