8061-E20
(Plasmid
#234257)
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PurposeGateway ORF Entry clone of human ACVR1C with stop codon (for native or N-terminal fusions)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234257 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonr-253
- Backbone size w/o insert (bp) 3155
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Vector typeGateway Entry Clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACVR1C
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Alt nameAlk7
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SpeciesH. sapiens (human)
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Insert Size (bp)1560
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Entrez GeneACVR1C (a.k.a. ACVRLK7, ALK7)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
- 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This gene contains 2 amino acid mutations compared with the human genome and human RefSEq sequences. However all available library clones contain these mutations. Unclear if these are true SNPs
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
8061-E20 was a gift from Dominic Esposito (Addgene plasmid # 234257 ; http://n2t.net/addgene:234257 ; RRID:Addgene_234257)