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Addgene

8061-E36
(Plasmid #234273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonr-253
  • Backbone size w/o insert (bp) 3155
  • Vector type
    Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1566
  • Entrez Gene
    Smad6 (a.k.a. Madh6, b2b390Clo)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
  • 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This gene contains 1 amino acid mutation from the RefSeq sequence. However, the C57Bl mouse genomic sequence matches the sequence here, suggesting the mutation is likely a SNP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8061-E36 was a gift from Dominic Esposito (Addgene plasmid # 234273 ; http://n2t.net/addgene:234273 ; RRID:Addgene_234273)
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