EML6428_ pRL662-eGFP
(Plasmid
#234350)
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PurposeExpressed eGFP(S65T) in agrobacteria for tracing in planta
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRL662-GFP(S65T)GentR
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpeR
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTCGATGTTTGATGTTATGGAGCAGCAACGATGGGGGAAGCGGTGATCGCCGAAGTATCG
- 3′ sequencing primer CGGGAAGCCGATCTCGGCTTGAACGAATTGTTATTTGCCGACTACCTTGGTGATCTCGCC
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EML6428_ pRL662-eGFP was a gift from Erh-Min Lai (Addgene plasmid # 234350 ; http://n2t.net/addgene:234350 ; RRID:Addgene_234350) -
For your References section:
Floral stage optimization and immune evasion enhance Agrobacterium-mediated genome editing in Arabidopsis. Liu MS, Huang TK, Wang YC, Wang SC, Wu CH, Kuo CH, Lai EM. New Phytol. 2025 Nov 30. doi: 10.1111/nph.70795. 10.1111/nph.70795 PubMed 41321035