EML6442_p35S-mCherry2-SV40
(Plasmid
#234351)
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PurposeExpressed mCherry2 florescent proteins in plant cells for tracing transformation events
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepICSL86922
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpeR
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SpeciesSynthetic
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer AAGGTCTCAAATGGTGAGTAAAGGAGAAGA
- 3′ sequencing primer AAGGTCTCAAAGCTTAGGCGCCTACCTTTCTCTTCTTTTTTGGGCCTTTGTATAGTTCATCCATGCCACC
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EML6442_p35S-mCherry2-SV40 was a gift from Erh-Min Lai (Addgene plasmid # 234351 ; http://n2t.net/addgene:234351 ; RRID:Addgene_234351) -
For your References section:
Floral stage optimization and immune evasion enhance Agrobacterium-mediated genome editing in Arabidopsis. Liu MS, Huang TK, Wang YC, Wang SC, Wu CH, Kuo CH, Lai EM. New Phytol. 2025 Nov 30. doi: 10.1111/nph.70795. 10.1111/nph.70795 PubMed 41321035