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pMDC32B-SlmiR482bTS-B/c
(Plasmid #234369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234369 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMDC32
  • Total vector size (bp) 11639
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    B/c
  • Alt name
    BsaI-ccdB
  • Insert Size (bp)
    1469

Gene/Insert 2

  • Gene/Insert name
    SlmiR482bTS
  • Alt name
    Solanum lycopersicum microRNA 482b target site
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    33

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDC32B-SlmiR482bTS-B/c was a gift from Alberto Carbonell (Addgene plasmid # 234369 ; http://n2t.net/addgene:234369 ; RRID:Addgene_234369)

  • For your References section:

    Precision RNAi in Tomato Using Synthetic Trans-Acting Small Interfering RNAs Derived From Minimal Precursors. Tomassi AH, Juarez-Molina M, Cisneros AE, Alarcia A, Orlando F, Toledano-Franco S, Presa S, Granell A, Carbonell A. Plant Biotechnol J. 2025 Oct 14. doi: 10.1111/pbi.70410. 10.1111/pbi.70410 PubMed 41085227
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