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Addgene

pNRCreBle
(Plasmid #234431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234431 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC
  • Backbone size w/o insert (bp) 2797
  • Total vector size (bp) 6350
  • Vector type
    Cre/Lox, CRISPR, Synthetic Biology ; Green algae Expression
  • Selectable markers
    Zeocin ; Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre recombinase
  • Insert Size (bp)
    1170
  • Mutation
    Picochlorum celeri endoglucanase intron insertion
  • GenBank ID
    AF330636
  • Promoter P celeri nitrate reductase

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Twist bioscience printed DNA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNRCreBle was a gift from Matthew Posewitz (Addgene plasmid # 234431 ; http://n2t.net/addgene:234431 ; RRID:Addgene_234431)

  • For your References section:

    Leveraging CRISPR Cas9 RNPs and Cre-loxP in Picochlorum celeri for generation of field deployable strains and selection marker recycling. Burch TA, Krishnan A, Posewitz MC. Front Microbiol. 2025 Jul 14;16:1588625. doi: 10.3389/fmicb.2025.1588625. eCollection 2025. 10.3389/fmicb.2025.1588625 PubMed 40727561
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