pRsetB-RoTq-Off
(Plasmid
#234466)
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PurposeBacterial expression vector for RoTq-Off
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234466 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRsetB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRoTq-Off
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Tag
/ Fusion Protein
- 7xHis (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRsetB-RoTq-Off was a gift from Gary Yellen (Addgene plasmid # 234466 ; http://n2t.net/addgene:234466 ; RRID:Addgene_234466) -
For your References section:
Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Rosen PC, Glaser A, Martinez-Francois JR, Lim DC, Brooks DJ, Fu P, Kim E, Kern D, Yellen G. Proc Natl Acad Sci U S A. 2025 May 13;122(19):e2503978122. doi: 10.1073/pnas.2503978122. Epub 2025 May 6. 10.1073/pnas.2503978122 PubMed 40327692