pGTM_Sumo2-N-8XHis-Plpro-C111S
(Plasmid #234472)

• Purpose: Expresses SARS-CoV-2 Nsp3 residues 746-1063 C111S with an activity abolishing mutation for the purification of this PLpro protease. Following Sumo Protease Cleavage an N-term 8xHis Tag is retained
• Depositing Lab: Gaetano Montelione
• Publication: De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. doi: 10.1016/j.xpro.2025.103952. Epub 2025 Jul 18.

Backbone

• Vector backbone: pET15Sumo2_NESG
• Backbone manufacturer: Novagen/Northeast Structural Genomics Consortium
• Backbone size w/o insert (bp): 5680
• Total vector size (bp): 6664
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PLpro
• Alt name: Nsp3
• Species: SARS-CoV-2
• Insert Size (bp): 984
• GenBank ID: WQM42602.1 PRO_0000449637
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: T7 lac
• Tags / Fusion Proteins:
  • 6xHis-Sumo (N terminal on backbone)
  • 8xHis (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry