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Addgene

pBHM2662
(Plasmid #234501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4887
  • Total vector size (bp) 9090
  • Vector type
    Bacterial Expression, Yeast Expression, CRISPR
  • Selectable markers
    URA3 ; acetamidase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen-CBP-2XFLAG-amdS-PCnU6-sgRNA_scaffold
  • Insert Size (bp)
    4264

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.14.638277 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHM2662 was a gift from Hiten Madhani (Addgene plasmid # 234501 ; http://n2t.net/addgene:234501 ; RRID:Addgene_234501)

  • For your References section:

    Near 100% efficient homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans. Nalley MJ, Banerjee S, Huang MY, Madhani HD. bioRxiv 2025.02.14.638277 10.1101/2025.02.14.638277
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