pAAV-cytoFLARE1.0/1.1-TF
(Plasmid #234516)

• Purpose: Transcriptional reporter for detecting calcium transients in neuronal cultures (transcription factor)
• Depositing Lab: Wenjing Wang
• Publication: Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):101012. doi: 10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ERT2-MK2-hLOV-TEVcs-FLAG-tTA
• Species: H. sapiens (human)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gcgaccatctgcgctgcggcgcc
• 3′ sequencing primer: cacatagcgtaaaaggagcaacatag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
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Depositor Comments

Please visit https://doi.org/10.1101/2025.01.13.632875 for bioRxiv preprint.