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Addgene

pUASTattB-cytoFLARE1.0-TF
(Plasmid #234521)

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Item Catalog # Description Quantity Price (USD)
Plasmid 234521 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUASTattB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ERT2-MK2-hLOV-TEVcs-FLAG-LexA-VP16
  • Species
    D. melanogaster (fly)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ccagcaaccaagtaaatcaactgcaac
  • 3′ sequencing primer ccttagagctttaaatctctgtaggtag
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.13.632875 for bioRxiv preprint.

There are some SNPs and indels in mini-white, the depositor confirms they are not of concern for plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTattB-cytoFLARE1.0-TF was a gift from Wenjing Wang (Addgene plasmid # 234521 ; http://n2t.net/addgene:234521 ; RRID:Addgene_234521)

  • For your References section:

    An improved FLARE system for recording and manipulating neuronal activity. Zhou G, Li R, Bartolik O, Ma Y, Wan WW, Meng J, Hu Y, Ye B, Wang W. bioRxiv [Preprint]. 2025 Jan 18:2025.01.13.632875. doi: 10.1101/2025.01.13.632875. 10.1101/2025.01.13.632875 PubMed 39868209
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