pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003
(Plasmid
#234546)
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Purposeto express dSpyCatcher on the extracellular side of astrocytic plasmamembrane
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-mGFAP(ABC1D)-*-WPRE-HBGpA
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameECS-dSC
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SpeciesOther
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MutationNone
- Promoter mGFAP(ABC1D)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.18.643859 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003 was a gift from Hajime Hirase (Addgene plasmid # 234546 ; http://n2t.net/addgene:234546 ; RRID:Addgene_234546) -
For your References section:
Functional and structural profiling of circulation via genetically encoded modular fluorescent probes. Vittani M, Lee AB, Wang X, Hiraoka Y, Konno A, Knak PG, Kusk P, Nagao M, Asiminas A, Courtin J, Putranto MF, Nasu Y, Tsuno S, Ueda K, Osuga Y, Tsuboi T, Bienvenu T, Terunuma M, Hirai H, Nedergaard M, Tanaka K, Hirase H. bioRxiv 2025.03.18.643859 10.1101/2025.03.18.643859