pAAV-mGFAP(ABC1D)-2pabPAC
(Plasmid
#234547)
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Purposeto express the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234547 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-mGFAP(ABC1D)-*-WPRE-HBGpA
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2pabPAC
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SpeciesOther
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MutationNone
- Promoter mGFAP(ABC1D)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-mGFAP(ABC1D)-2pabPAC was a gift from Hajime Hirase (Addgene plasmid # 234547 ; http://n2t.net/addgene:234547 ; RRID:Addgene_234547) -
For your References section:
Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP(3)R2-mediated Ca(2+) signaling in mice. Vittani M, Herlo R, Wang X, Christensen MDB, Vo CT, Mishima T, Kusk P, Konno A, Hirai H, Tsuboi T, Kitaguchi T, Kjaerby C, Asiminas A, Yokoyama T, Sakamoto M, Nedergaard M, Hirase H. Proc Natl Acad Sci U S A. 2025 Jul 8;122(27):e2422069122. doi: 10.1073/pnas.2422069122. Epub 2025 Jul 1. 10.1073/pnas.2422069122 PubMed 40591593