B95 ΔAΔfabRΔselABC
(Bacterial strain
#234550)
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PurposeThis strain is used for expressing selenocysteine-containing proteins using plasmid pSecUAG-UTu1D
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Depositing Labs
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 234550 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)B95 ΔAΔfabRΔselABC
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Growth instructions16–37 °C
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genotype: The same as BL21(DE3) except for the additional mutations at 95 UAG codons, disruption of prfA, and frameshift in fabR and deletion of selABC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
B95 ΔAΔfabRΔselABC was a gift from Natalie Krahn & Dieter Söll (Addgene plasmid # 234550) -
For your References section:
Intein-based Design Expands Diversity of Selenocysteine Reporters. Chung CZ, Krahn N, Crnkovic A, Soll D. J Mol Biol. 2022 Apr 30;434(8):167199. doi: 10.1016/j.jmb.2021.167199. Epub 2021 Aug 16. 10.1016/j.jmb.2021.167199 PubMed 34411545